Microplants production of Eucalyptus cloeziana from indirect organogenesis

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Published: Jul 31, 2020
DOI: https://doi.org/10.4067/S0717-92002020000200113
Keywords:
- adventitious rooting
- callogenesis
- indirect regeneration
- adventitious bud

Main Article Content

Alex Zichner Zorz
Federal University of Mato Grosso (UFMT), College of Forestry, Cuiabá, Brazil.
Júlio Cézar Tannure Faria
Federal University of Lavras (UFLA), Department of Forestry Sciences, Lavras, MG, Brazil.
Denys Matheus Santana Costa Souza
Federal University of Lavras (UFLA), Department of Forestry Sciences, Lavras, MG, Brazil.
Douglas Santos Gonçalves
Federal University of Lavras (UFLA), Department of Forestry Sciences, Lavras, MG, Brazil.
Leandro Silva de Oliveira
Federal University of Minas Gerais (UFMG), Institute of Agronomic Sciences, Montes Claros, MG, Brazil.
André Luís Lopes Da Silva
State University of Bahia, Department of Technology and Social Sciences, Juazeiro, BA, Brazil.
Wellington Ferreira Campos
Federal University of Jequitinhonha e Mucuri Valleys (UFVJM), Institute of Agronomic Sciences, Unaí, MG, Brazil.
Gilvano Ebling Brondani
Federal University of Lavras (UFLA), Department of Forestry Sciences, Lavras, MG, Brazil.

Abstract

Few studies have focused on the in vitro multiplication methods for Eucalyptus cloeziana. In this work we developed an indirect organogenesis protocol to obtain micropropagated plants. The interactions of plant growth regulators in juvenile tissues were evaluated. Hypocotyl, cotyledon and root tissues obtained from in vitro germinated seedlings were subjected to combinations of α-naphthaleneacetic acid (NAA), thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D). In vitro callogenesis, adventitious bud induction, shoot elongation, and ex vitro survival and rooting were evaluated. Callogenic structures were observed in all evaluated tissues. Morphogenic characteristics related to the meristematic center was observed. The tissues that presented the callus mass were subcultured in a regeneration culture medium supplemented with 1.0 mg L-1 of 6-benzylaminopurine (BAP). Only hypocotyl and cotyledon induced adventitious bud and shoot elongation. Hypocotyl subjected to 2.0 mg L-1 TDZ had the highest number of elongated shoots. The two tissues (i.e., hypocotyl and cotyledon) combined with thidiazuron were characterized by the ex vitro survival of microcuttings and by adventitious rooting. Results confirmed tissue culture efficiency for microplants production by indirect organogenesis from hypocotyl and cotyledon cultivated with TDZ, and its implementation can be an alternative for forest tree breeding programs of E. cloeziana.

Article Details

How to Cite
Zichner Zorz, A., Tannure Faria, J. C., Santana Costa Souza, D. M., Santos Gonçalves, D., Silva de Oliveira, L., Lopes Da Silva, A. L., Ferreira Campos, W., & Ebling Brondani, G. (2020). Microplants production of Eucalyptus cloeziana from indirect organogenesis. Bosque, 41(2), 113–124. https://doi.org/10.4067/S0717-92002020000200113
Issue
Vol. 41 No. 2 (2020)
Section
Articles
Author Biographies

Alex Zichner Zorz, Federal University of Mato Grosso (UFMT), College of Forestry, Cuiabá, Brazil.

Federal University of Mato Grosso (UFMT), College of Forestry, Fernando Corrêa da Costa Avenue, 2367, Cuiabá, MT, 78060-900, Brazil.

Júlio Cézar Tannure Faria, Federal University of Lavras (UFLA), Department of Forestry Sciences, Lavras, MG, Brazil.

Federal University of Lavras (UFLA), Department of Forestry Sciences, P.O. Box 3037, 37200-900, Lavras, MG, Brazil, [email protected] or [email protected]

Denys Matheus Santana Costa Souza, Federal University of Lavras (UFLA), Department of Forestry Sciences, Lavras, MG, Brazil.

Federal University of Lavras (UFLA), Department of Forestry Sciences, P.O. Box 3037, 37200-900, Lavras, MG, Brazil, [email protected] or [email protected]

Douglas Santos Gonçalves, Federal University of Lavras (UFLA), Department of Forestry Sciences, Lavras, MG, Brazil.

Federal University of Lavras (UFLA), Department of Forestry Sciences, P.O. Box 3037, 37200-900, Lavras, MG, Brazil, [email protected] or [email protected]

Leandro Silva de Oliveira, Federal University of Minas Gerais (UFMG), Institute of Agronomic Sciences, Montes Claros, MG, Brazil.

Federal University of Minas Gerais (UFMG), Institute of Agronomic Sciences, Universitária Avenue, n.1000, 39404-547, Montes Claros, MG, Brazil.

André Luís Lopes Da Silva, State University of Bahia, Department of Technology and Social Sciences, Juazeiro, BA, Brazil.

State University of Bahia, Department of Technology and Social Sciences, Edgar Chastinet, s/n, 48905-680, Juazeiro, BA, Brazil.

Wellington Ferreira Campos, Federal University of Jequitinhonha e Mucuri Valleys (UFVJM), Institute of Agronomic Sciences, Unaí, MG, Brazil.

Federal University of Jequitinhonha e Mucuri Valleys (UFVJM), Institute of Agronomic Sciences, Vereador João Narciso Avenue, n.1380, 38610-000, Unaí, MG, Brazil.

Gilvano Ebling Brondani, Federal University of Lavras (UFLA), Department of Forestry Sciences, Lavras, MG, Brazil.

Federal University of Lavras (UFLA), Department of Forestry Sciences, P.O. Box 3037, 37200-900, Lavras, MG, Brazil, [email protected] or [email protected]

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