First report of isolation of Staphylococcus schleiferi subspecies coagulans from dogs with pyoderma and external otitis in Chile

Abstract

Staphylococcus schleiferi subsp. coagulans was identified and described for the first time in 1990 by Igimi et al(1990) in dogs with otitis. The objective of this report was to determine the frequency of isolation of S. schleiferisubsp. coagulans in samples from dogs with pyoderma and / or otitis externa in Chile and establish the resistance profiles to commonly used antimicrobials and molecularly characterize the quinolone resistance. Samples were obtained from 237 adult dogs diagnosed with otitis externa and/or pyoderma, at the Clinical Veterinary Hospital of the University of Chile. The identification of S. schleiferi subsp. coagulans was performed with BBL™ Crystal GP. The minimum inhibitory concentration was determined using the agar diffusion method. The antimicrobial panel contained oxacillin, amoxicillin, cefradine, mupirocyn, clindamycin, enrofloxacin, doxycycline, tetracycline, kanamycin and sulfamethoxazole / trimethoprim. 135 out of 237 total samples analyzed were isolated from pyoderma and 102 from otitis. Staphylococcus spp. was identified on 117 and 52 of the samples, respectively. S. schleiferi subsp. coagulans was identified on 8 strains isolated from pyoderma (6.8%) and on 11 strains from otitis (21.2%). All strains of S. schleiferi subsp. coagulans were from pyoderma and 54.5% of the otitis strains were susceptible to the antimicrobial agents. The susceptibility was 90.9% to mupirocyn, enrofloxacin and sulfamethoxazole/trimethoprim; 81.8% to amoxicillin and kanamycin and 72.3% to clindamycin. One strain isolated from otitis was multi-resistant. The strain of Staphylococcus schleiferi subsp. coagulans that showed phemotypic resistance to quinolones reported mutations in genes gyrA and grlA.