Evaluation of a double centrifugation tube method for concentrating bovine platelets: cellular study

Abstract

The aim of this study was to evaluate a double centrifugation tube method for concentrating bovine platelets. Whole blood samples were collected from clinically normal Holstein cows and processed using a double centrifugation tube method to obtain platelet rich plasma (PRP). PRP and whole blood were analysed by a volumetric impedance system for hemogram and additional platelet parameters (mean platelet volume (MPV) and platelet distribution width (PDW). Platelet concentrations for PRP were 229% higher in comparison with platelet concentration for whole blood samples. There were positive significant correlations between platelet and lymphocyte counts (ρ = 0.81; P = 0.0001), platelet and leukocyte counts (ρ = 0.75; P = 0.0001) and, a significant negative correlation between platelet count and PDW (ρ = –0.72; P = 0.0001). In conclusion, the double centrifugation tube method evaluated in this study is a reliable method for producing bovine PRP.