Immunohistochemical methods for the identification of enteropathogenic E. coli fimbriae
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Abstract
A study to evaluate two Inmunohistochemistry (IHC) techniques (Indirect Immunoflourescense IFI and Streptavidina-Biotine-Peroxidase LSAB) to identify the antigen fimbrial of different enteropathogenic strains of E. coli was done, correlating the results with the Aglutination test.
Newborn piglets without calostrum were innoculated with enteropathogenic strains of E. coli which contained F4, F5, F6 and F41. Piglets were analysed to microbiologically isolate and identify the antigen fimbrial in the small intestine with IHC and Aglutination test.
The IHC techniques , identified F4 fimbriae was in the three intestinal segments ; However, the Ag F5, F6 and F41 were found in the ileon and jejunum with lesser frequency than in the duodenum. The aglutination test identified antigen F4 and F5 but was not able to identify F6 and F41.
These results suggest that IFI in cryostat-microtome sections and LSAB in paraffin embedded tissues, are effective and may be useful for a rapid analysis and for epidemiological studies of suckling pigs with diahrroea for E. coli .